Differences

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--- cbi-e2018 [2024/03/08 15:16] – created Luc Bonnefond
+++ cbi-e2018 [2024/03/08 16:51] (current) – Luc Bonnefond
@@ Line 8: / Line 8: @@
   * [[https://seafile.igbmc.fr/library/Lamour-Ruff_Common/|Prod Schedule for Media Order_2024]]
 Please **fill the green lines** in this table. All the rest will be calculated automatically, allowing Julien to follow the remaining stocks.
+{{:e2018_media_usage.png?600|}}
 ==== How to book the incubators ====
@@ Line 20: / Line 22: @@
   * **Good to know**: Julien has a « team » stock for « luna counting » slides and adhesive membranes (breathseal sealer). No need to order yourself, just go ask him.
+----
+==== Reminder for media ====
+=== Liquid GMEM ===
+{{::gmem-bottle.jpg?100 |}}500 mL
+  * + 30 mL BTP 30g/L (0,22µ filtred under PSM)
+  * + 50 mL SVF
+  * + 150 µL Gentamycin
+=== GMEM powder ===
+{{:gmem-vial.jpg?100|}} **vial for 1 L** or **bottle for 10 L** {{:gmem-bottle.jpg?100|}}
+vial for 1 L culture: (or 1 bottle (for 10 L) weight up what you need)
+  * Re-suspend in 900 mL sterilized mQ water (Denis)
+  * + 60 mL BTP
+  * + 30 mL Sodium Bicarbonate 90 g/L
+  * + 100 mL SVF
+  * + 300 µL Gentamycin
+{{:sodium_bicarbonate.jpg?100 |}}**Sodium Bicarbonate** :
+  * warm to 25°C for complete dissolution
+  * 0,22 µ filtered under PSM
+----
+==== Cleaning ====
+Please clean the PSM before and after your work with ummonium spray!
+Don’t let your dirty bottles more than one day for decontamination (1 week-end, not more)
+Cleaning sessions (each 3 weeks) has been  scheduled by Mylene
+[[https://docs.google.com/spreadsheets/d/1f-fB5N8KR6xmudWqZ6yslXfAFT8ivqy3OuFtUXROMNs/edit?usp=sharing|schedule]]
+{{:e2018-cleaning_schedule_202403.png|}}