cloning:gatc
Differences
This shows you the differences between two versions of the page.
| Next revision | Previous revision | ||
| cloning:gatc [2017/02/23 09:15] – created bonnefon | cloning:gatc [2023/11/01 20:18] (current) – external edit 127.0.0.1 | ||
|---|---|---|---|
| Line 26: | Line 26: | ||
| The melting temperature (TM) of the primer should be between 52°and 58°C and the length should be between 17-19bp. Ideally, the GC content should be 10 G+C for a 17mer; 8-9G+C for an 18mer and 7-9 G+C for a 19mer. | The melting temperature (TM) of the primer should be between 52°and 58°C and the length should be between 17-19bp. Ideally, the GC content should be 10 G+C for a 17mer; 8-9G+C for an 18mer and 7-9 G+C for a 19mer. | ||
| G or C should be at the 3’ end, but not more than 3 Gs or Cs. The primer sequence should be a good mix of all 4 nucleotides with no more than 4 identical bases in a row (AAAA or GGGG). | G or C should be at the 3’ end, but not more than 3 Gs or Cs. The primer sequence should be a good mix of all 4 nucleotides with no more than 4 identical bases in a row (AAAA or GGGG). | ||
| + | |||
| + | {{ : | ||
| **Storage of DNA and primer** | **Storage of DNA and primer** | ||
| Line 66: | Line 68: | ||
| //You will be supported | //You will be supported | ||
| - | |||
| - | |||
cloning/gatc.1487841303.txt.gz · Last modified: (external edit)