Differences

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--- cryosparc [2024/02/12 09:04] – Luc Bonnefond
+++ cryosparc [2024/08/07 15:51] (current) – Luc Bonnefond
@@ Line 1: / Line 1: @@
 ====== using cryoSPARC ======
-===== modify the particles.star from Warp =====
+===== modify the particles.star file from Warp =====
 The particles.star files generated by Warp during the on-the-fly processing link each particle to its tif movie. To import the particles in cryoSPARC and keep the link with the motion corrected micrographs, the star file must be edited.
@@ Line 10: / Line 10: @@
 <code>
 :
-%s/ <first_characters_of_movie_files> \/average/<first_characters_of_movie_files>
+%s/ <first_characters_of_movie_files>/ average\/<first_characters_of_movie_files>
 </code>
@@ Line 32: / Line 32: @@
+----
+===== Reduce the size of a particle stack subset =====
+To reduce the size of the space required on the SSD cache if you are working with a subset of the full extracted particles stack (after selecting a subset of particles with <quote>2D classification</quote> job for example)
+  * Run a <quote>Downsample Particles</quote> job on the subset (output of <quote>select 2D</quote> job in my previous example) without specifying any downsampling.
+The output can be used for further processing and will be smaller and thus faster to load on the scratch. Otherwise the full stask will be loaded in the scratch event thoush only part of it will be used.
+----
 ===== Generate a defect file for cryoSPARC =====
@@ Line 195: / Line 203: @@
 Digital Micrograph(TM), GMS v 3.32
 SUBIMAGE: Subtract section B from section A.            28-Oct-21  11:42:19
 </code>