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crystallography:collection:frx:usage

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crystallography:collection:frx:usage [2016/07/12 09:26] – bonnefoncrystallography:collection:frx:usage [2023/11/01 20:19] (current) – external edit 127.0.0.1
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 ====== Rigaku FR-X Usage ====== ====== Rigaku FR-X Usage ======
- 
- 
  
 Training on 2015.03.30 Training on 2015.03.30
  
-FR-X generator: 45 kV, 66 mA, 70 μ cross-section, 180 μ beam, 1.7 miliradians divergence+**FR-X generator**:  
 +  * 45 kV,  
 +  * 66 mA,  
 +  * 70 μ cross-section,  
 +  * 180 μ beam,  
 +  * 1.7 miliradians divergence
  
 **XG Control** tells you the settings values (45 kV and 66 mA vs 20 kV and 10 mA when not used) .  **XG Control** tells you the settings values (45 kV and 66 mA vs 20 kV and 10 mA when not used) . 
 You can screen as soon as the parameters have reached the target values, but wait one more hour to collect, so that the beam is stable. You can screen as soon as the parameters have reached the target values, but wait one more hour to collect, so that the beam is stable.
  
-Pilatus 300K detector: 3 chips, shorter exposure time, no readout (no shutter closing), almost never overloaded (>1.5 millions), water circulation at room temperature, dry air+**Pilatus 300K detector**:  
 +  * 3 chips,  
 +  * shorter exposure time,  
 +  * no readout (no shutter closing),  
 +  * almost never overloaded (>1.5 millions),  
 +  * water circulation at room temperature,  
 +  * dry air
  
 <code>>thread</code> command on camserver gives the temperature and humidity level <code>>thread</code> command on camserver gives the temperature and humidity level
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 **Phase**: **Phase**:
-High resolution limit; try different resolution cutoff+High resolution limit; try different resolution cutoffs
    
 **Check hand** (//ShelXE//): **Check hand** (//ShelXE//):
-You should see the difference, darkred/dark blue, difference of one graduation in scale+You should see the difference, dark red/dark blue, difference of one graduation in scale. 
 +If necessary, increase the cycle number so that a plateau is reached.
  
 **Phase**: **Phase**:
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 **NCS**: **NCS**:
 +//DM// or //Parrot// (a bit better)
 At least try it if you have more than 1 molecule in the Asymmetric Unit At least try it if you have more than 1 molecule in the Asymmetric Unit
  
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 //ARP/WARP// for high resolution //ARP/WARP// for high resolution
 //Buccaner// for lower resolution (∼ 3 Å) //Buccaner// for lower resolution (∼ 3 Å)
 +Let ARP/WARP build/refine several times if has difficulties building. Start several times witha few cycles rather that once with many cycles.
  
  
crystallography/collection/frx/usage.1468315579.txt.gz · Last modified: (external edit)