glacios_alignment
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| glacios_alignment [2022/11/18 13:43] – duranda | glacios_alignment [2023/11/01 20:18] (current) – external edit 127.0.0.1 | ||
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| ==== Objective Astigmatism / Coma-free alignment ==== | ==== Objective Astigmatism / Coma-free alignment ==== | ||
| - | This is done in SerialEM. You need to have a sample inserted to do it. Re-use the calibration specimen or a carbon Quantifoil (not a gold grid!) | + | This is done in SerialEM. You need to have a sample inserted to do it. Re-use the calibration specimen or a carbon Quantifoil (not a gold grid!). Use the Imaging state you will use for data collection (e.g. mag 45.000kx, spot 6, C2 aperture 30 µm, C2 lens 39.6%) |
| - Insert and center Objective Aperture | - Insert and center Objective Aperture | ||
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