glacios_collect
Differences
This shows you the differences between two versions of the page.
| Both sides previous revisionPrevious revisionNext revision | Previous revision | ||
| glacios_collect [2022/02/11 15:24] – bonnefon | glacios_collect [2023/11/01 20:18] (current) – external edit 127.0.0.1 | ||
|---|---|---|---|
| Line 111: | Line 111: | ||
| </ | </ | ||
| - | * Introduce the Objective aperture | + | Introduce the Objective aperture |
| - | * Redo the Correct astigmatism by CTF step once | + | |
| + | ---- | ||
| + | |||
| + | ==== Configuration of the multihole / multishot acquisition ==== | ||
| + | |||
| + | Do this configuration just before starting the acquisition on one of the squares selected for the whole acquisition after correction of the image shift in x280 and LD mode imaging states. | ||
| + | |||
| + | * On the square map, mark the positions of the 4 holes at the corners of a 9 holes square. | ||
| + | * Go to the center hole with **Go to marker** and properly center on the hole with **Stage shift**. | ||
| + | * Press View in LD mode (ensure that defocus is below -20) | ||
| + | * Open Menu: // | ||
| + | * Change the parameters of the multishot for calibration | ||
| + | * Click on the central hole for positioning | ||
| + | * Adjust the number of shots per hole (in general 3) | ||
| + | * Adjust the distance (72 in general for R 2/2 grids) | ||
| + | * Menu: // | ||
| + | * Select regular pattern 3 x 3 | ||
| + | * ++Calibrate after ensuring proper centering on the center hole|< | ||
| + | * Move by Image shift to the first corner | ||
| + | * Center properly in the hole using zoom if necessary | ||
| + | * Save the position by clicking on **Save image shift** | ||
| + | * Proceed to the next corner and repeat | ||
| + | </ | ||
| + | * Save the settings in //Menu > Settings > Save// | ||
| + | |||
| + | == Beam tuning == | ||
| + | |||
| + | * Ensure that LD modes View and Record are perfectly aligned | ||
| + | * Calibrate the proper centering in Record/ | ||
| + | |||
| + | * Precisely center Record beam with Direct Alignments/ | ||
| + | * Check **Continuous update** | ||
| + | * Press View and recenter if necessary with set add beam shift | ||
| + | * ++Then cycle several times between the modes until the beam stop moving|< | ||
| + | * Press **Record** and correct Direct Alignments/ | ||
| + | * Press **Focus** and set add beam shift | ||
| + | * Press **Trial** and set add beam shift | ||
| + | * Repeat | ||
| + | </ | ||
| + | * Uncheck **Continuous update** | ||
glacios_collect.1644593065.txt.gz · Last modified: (external edit)