glacios_interface
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| glacios_interface [2022/02/11 13:02] – created bonnefon | glacios_interface [2023/11/01 20:18] (current) – external edit 127.0.0.1 | ||
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| - | ====== The microscope console | + | ===== The microscope console ===== |
| **Location**: | **Location**: | ||
| Line 14: | Line 14: | ||
| * and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs. | * and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs. | ||
| - | ====== Gatan DigitalMicrograph (DM) ====== | + | ---- |
| - | **Location**: | + | ===== Gatan DigitalMicrograph User Interface (DM) ===== |
| - | **Role**: it controls | + | **Location**: On the left screen |
| - | The beginner user will not use it. ++The expert user will use it to: | + | **Role**: It controls the K2 direct electron detector. |
| + | |||
| + | * The beginner user will not use it. | ||
| + | * ++The expert user will use it to: | ||
| * prepare Dark, linear and counting / Super Resolution gain references. | * prepare Dark, linear and counting / Super Resolution gain references. | ||
| * measure the dose. | * measure the dose. | ||
| Line 27: | Line 30: | ||
| * perform cryo-cycle of the detector | * perform cryo-cycle of the detector | ||
| </ | </ | ||
| + | |||
| + | ---- | ||
| + | |||
| + | ===== SerialEM User Interface (SEM) ===== | ||
| + | |||
| + | **Location**: | ||
| + | |||
| + | **Role**: It provides camera/ | ||
| + | |||
| + | * ++The beginner user will use it to: | ||
| + | * record the Atlases. | ||
| + | * select squares and prepare the square maps at eucentric height. | ||
| + | * select targets (holes). | ||
| + | * screen targets (holes). | ||
| + | </ | ||
| + | * ++The expert user will use it to: | ||
| + | * perform all the actions described above. | ||
| + | * perform beam corrections (astigmatism, | ||
| + | * perform calibrations. | ||
| + | * setup the dose rate, total dose and dose fractionning. | ||
| + | * setup the time and imaging modes for the different beam modes. | ||
| + | * center the different beam modes all together on the camera axis. | ||
| + | </ | ||
| + | |||
| + | ---- | ||
| + | |||
| + | ===== The TEM User Interface (TEM) ===== | ||
| + | |||
| + | **Location**: | ||
| + | |||
| + | **Role**: It controls all the microscope systems. | ||
| + | |||
| + | * ++The beginner user will use it to: | ||
| + | * dock/undock the cassette from the NanoCab to the autoloader. | ||
| + | * load/unload the grids in the column. | ||
| + | * change and center the Condenser 2 and objective apertures. | ||
| + | * control the autoloader turbo pump. | ||
| + | * perform beam-shift adjustments. | ||
| + | * manually set eucentric height. | ||
| + | </ | ||
| + | * ++The expert user will use it to: | ||
| + | * perform all the actions described above. | ||
| + | * align the microscope. | ||
| + | * troubleshoot the system. | ||
| + | * manage the cold system. | ||
| + | * perform cryo-cycle of the column/ | ||
| + | </ | ||
| + | |||
| + | ---- | ||
| + | |||
| + | ===== The FluCam User Interface (FC) ===== | ||
| + | |||
| + | **Location**: | ||
| + | |||
| + | **Role**: It allows the visualization of the electron beam and the sample image. | ||
| + | |||
| + | * ++The beginner user will use it to: | ||
| + | * center the beams. | ||
| + | * center Condenser 2 and objective apertures. | ||
| + | * look for and move to particular features on their grids. | ||
| + | * manually set eucentric height. | ||
| + | </ | ||
| + | * ++The expert user will use it to: | ||
| + | * perform all the actions described above. | ||
| + | * align the microscope. | ||
| + | </ | ||
| + | |||
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