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glacios_interface [2022/02/11 13:02] – created bonnefonglacios_interface [2023/11/01 20:18] (current) – external edit 127.0.0.1
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-====== The microscope console ======+===== The microscope console =====
  
 **Location**: right in front of you on the desk **Location**: right in front of you on the desk
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   * and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs.    * and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs. 
  
-====== Gatan DigitalMicrograph (DM) ======+----
  
-**Location**: on the left screen+===== Gatan DigitalMicrograph User Interface (DM) =====
  
-**Role**: it controls the K2 direct electron detector+**Location**: On the left screen
  
-The beginner user will not use it. ++The expert user will use it to:|<div>+**Role**: It controls the K2 direct electron detector. 
 + 
 +  * The beginner user will not use it. 
 +  * ++The expert user will use it to:|<div>
   * prepare Dark, linear and counting / Super Resolution gain references.   * prepare Dark, linear and counting / Super Resolution gain references.
   * measure the dose.   * measure the dose.
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   * perform cryo-cycle of the detector   * perform cryo-cycle of the detector
 </div>++ </div>++
 +
 +----
 +
 +===== SerialEM User Interface (SEM) =====
 +
 +**Location**: On the left screen
 +
 +**Role**: It provides camera/microscope commands to allow the user to screen its grids and setup an automated data collection.
 +
 +  * ++The beginner user will use it to:|<div>
 +  * record the Atlases.
 +  * select squares and prepare the square maps at eucentric height.
 +  * select targets (holes).
 +  * screen targets (holes).
 +</div>++
 +  * ++The expert user will use it to:|<div>
 +  * perform all the actions described above.
 +  * perform beam corrections (astigmatism, coma-free).
 +  * perform calibrations.
 +  * setup the dose rate, total dose and dose fractionning.
 +  * setup the time and imaging modes for the different beam modes.
 +  * center the different beam modes all together on the camera axis.
 +</div>++
 +
 +----
 +
 +===== The TEM User Interface (TEM) =====
 +
 +**Location**: On the middle screen
 +
 +**Role**: It controls all the microscope systems.
 +
 +  * ++The beginner user will use it to:|<div>
 +  * dock/undock the cassette from the NanoCab to the autoloader.
 +  * load/unload the grids in the column.
 +  * change and center the Condenser 2 and objective apertures.
 +  * control the autoloader turbo pump.
 +  * perform beam-shift adjustments.
 +  * manually set eucentric height.
 +</div>++
 +  * ++The expert user will use it to:|<div>
 +  * perform all the actions described above.
 +  * align the microscope.
 +  * troubleshoot the system.
 +  * manage the cold system.
 +  * perform cryo-cycle of the column/autoloader.
 +</div>++
 +
 +----
 +
 +===== The FluCam User Interface (FC) =====
 +
 +**Location**: On the right screen
 +
 +**Role**: It allows the visualization of the electron beam and the sample image.
 +
 +  * ++The beginner user will use it to:|<div>
 +  * center the beams.
 +  * center Condenser 2 and objective apertures.
 +  * look for and move to particular features on their grids.
 +  * manually set eucentric height.
 +</div>++
 +  * ++The expert user will use it to:|<div>
 +  * perform all the actions described above.
 +  * align the microscope.
 +</div>++
 +
glacios_interface.1644584549.txt.gz · Last modified: (external edit)