BSI wiki

User Tools

Site Tools

Trace: • c_protocol
glacios_screening

Differences

This shows you the differences between two versions of the page.

Link to this comparison view

glacios_screening [2023/01/16 14:13] – marechanglacios_screening [2023/11/01 20:18] (current) – external edit 127.0.0.1
Line 4: Line 4:
  
  
-==== 1. Load the Grids ====+==== 1. Load  Grid ====
  
   - ++Introduce your grids in the cassette|<div>   - ++Introduce your grids in the cassette|<div>
Line 13: Line 13:
   - ++Check Autoloder temperatures, then load the NanoCab in the microscope|<div>{{ :autoloader_tempertures_all.png?nolink |}}   - ++Check Autoloder temperatures, then load the NanoCab in the microscope|<div>{{ :autoloader_tempertures_all.png?nolink |}}
 </div>++ </div>++
-  - Remove the NanoCab+ 
 + 
 + 
  
  
Line 33: Line 36:
 </div>++ </div>++
   - ++Load platform or user settings|<div>   - ++Load platform or user settings|<div>
-  * Menu: //Settings > Open > SerialEM_Settings_SPA//+  * Menu: //Settings > Open > Platform_Settings_SPA//
 </div>++  </div>++ 
  
Line 100: Line 103:
     * On the microscope computer, make sure beam settings were updated according to Atlas imaging state.     * On the microscope computer, make sure beam settings were updated according to Atlas imaging state.
 </div>++  </div>++ 
 +  - Open the column valves.
   - ++(Optional) Determine the overall eucentric height.|<div>   - ++(Optional) Determine the overall eucentric height.|<div>
     * In Serial-EM, center a square in the middle of the grid.     * In Serial-EM, center a square in the middle of the grid.
       - add a marker (green cross) by **left-clicking** on a square.       - add a marker (green cross) by **left-clicking** on a square.
       - in the navigator **windows** press the //go to marker// button. Wait for the stage to reach the specified position.       - in the navigator **windows** press the //go to marker// button. Wait for the stage to reach the specified position.
-      - take a //record// image.+      - take a //record// image.{{ :camera_and_script_panel_record.png?nolink&400 |}}
       - if the square is off-center: keep pressed the right mouse button, grab the center of the square to the center of the screen, and release the button. Wait for the stage to reach its position.       - if the square is off-center: keep pressed the right mouse button, grab the center of the square to the center of the screen, and release the button. Wait for the stage to reach its position.
      * In //Tasks// **tab** > //Eucentricity// > press on //Rough Eucentricity//.      * In //Tasks// **tab** > //Eucentricity// > press on //Rough Eucentricity//.
Line 192: Line 196:
     * If you did a mistake, like applying the shift with an atlas/square map selected instead of the point, the sift can be deleted in Navigator **tab** > //Undo last Shift//.     * If you did a mistake, like applying the shift with an atlas/square map selected instead of the point, the sift can be deleted in Navigator **tab** > //Undo last Shift//.
 </div>++ </div>++
 +  - Correct for coordinate discrepancy between View and Record Magnifications.
   - ++Select the focusing area|<div>   - ++Select the focusing area|<div>
   - Center a hole. If you plan to record images at the hole periphery, center the camera closer to a hole edge.   - Center a hole. If you plan to record images at the hole periphery, center the camera closer to a hole edge.
Line 237: Line 242:
   - With **multifunction X & Y knobs**, center the beam on the fluorescent screen, then press //Done//.   - With **multifunction X & Y knobs**, center the beam on the fluorescent screen, then press //Done//.
   - Switch to **View beam** with the //Vie.// button. Tick //Set// in //Additional beam shift//. **View beam only need to be centered once**.   - Switch to **View beam** with the //Vie.// button. Tick //Set// in //Additional beam shift//. **View beam only need to be centered once**.
-  - With the **trackball**, center the view beam on the fluorescent screen.+  - With the **trackball**, center the view beam on the fluorescent screen. **Untick** the //Set// button.
   - Center the Record beam again as described above.   - Center the Record beam again as described above.
   - Switch to **Focus beam** (//Foc.// button) and change its diameter with the intensity knob according with the desired focus surface (depends on your hole spacing).   - Switch to **Focus beam** (//Foc.// button) and change its diameter with the intensity knob according with the desired focus surface (depends on your hole spacing).
glacios_screening.1673878394.txt.gz · Last modified: (external edit)