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glacios_screening [2023/01/17 13:27] – [5. Record square maps] marechanglacios_screening [2023/11/01 20:18] (current) – external edit 127.0.0.1
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-==== 1. Load the Grids ====+==== 1. Load  Grid ====
  
   - ++Introduce your grids in the cassette|<div>   - ++Introduce your grids in the cassette|<div>
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   - ++Check Autoloder temperatures, then load the NanoCab in the microscope|<div>{{ :autoloader_tempertures_all.png?nolink |}}   - ++Check Autoloder temperatures, then load the NanoCab in the microscope|<div>{{ :autoloader_tempertures_all.png?nolink |}}
 </div>++ </div>++
-  - Remove the NanoCab+ 
 + 
 + 
  
  
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 </div>++ </div>++
   - ++Load platform or user settings|<div>   - ++Load platform or user settings|<div>
-  * Menu: //Settings > Open > SerialEM_Settings_SPA//+  * Menu: //Settings > Open > Platform_Settings_SPA//
 </div>++  </div>++ 
  
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     * If you did a mistake, like applying the shift with an atlas/square map selected instead of the point, the sift can be deleted in Navigator **tab** > //Undo last Shift//.     * If you did a mistake, like applying the shift with an atlas/square map selected instead of the point, the sift can be deleted in Navigator **tab** > //Undo last Shift//.
 </div>++ </div>++
 +  - Correct for coordinate discrepancy between View and Record Magnifications.
   - ++Select the focusing area|<div>   - ++Select the focusing area|<div>
   - Center a hole. If you plan to record images at the hole periphery, center the camera closer to a hole edge.   - Center a hole. If you plan to record images at the hole periphery, center the camera closer to a hole edge.
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   - With **multifunction X & Y knobs**, center the beam on the fluorescent screen, then press //Done//.   - With **multifunction X & Y knobs**, center the beam on the fluorescent screen, then press //Done//.
   - Switch to **View beam** with the //Vie.// button. Tick //Set// in //Additional beam shift//. **View beam only need to be centered once**.   - Switch to **View beam** with the //Vie.// button. Tick //Set// in //Additional beam shift//. **View beam only need to be centered once**.
-  - With the **trackball**, center the view beam on the fluorescent screen.+  - With the **trackball**, center the view beam on the fluorescent screen. **Untick** the //Set// button.
   - Center the Record beam again as described above.   - Center the Record beam again as described above.
   - Switch to **Focus beam** (//Foc.// button) and change its diameter with the intensity knob according with the desired focus surface (depends on your hole spacing).   - Switch to **Focus beam** (//Foc.// button) and change its diameter with the intensity knob according with the desired focus surface (depends on your hole spacing).
glacios_screening.1673962032.txt.gz · Last modified: (external edit)