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purification:tev [2016/07/13 15:04] – bonnefonpurification:tev [2023/11/01 20:19] (current) – external edit 127.0.0.1
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 ===== In-house TEV production ===== ===== In-house TEV production =====
  
 +----
  
-[[https://www.addgene.org/8827/|pRK792 plasmid]] - MBP-TEV, S219V mutant+==== Clones ==== 
 + 
 +[[https://www.addgene.org/8827/|pRK792 plasmid]] - His-TEV(S219V)-Arg - Waugh 
 + 
 +**Sequence**  
 +<q>G**HHHHHHH**GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFM__V__KPEEPFQPVKEATQLMN**RRRRR**</q> 
 + 
 +His-TEV(L35V/S135G/S219V)-Arg - Cabrita(([[http://onlinelibrary.wiley.com/doi/10.1110/ps.072822507/abstract;jsessionid=4269BED8C9677045F562FB78F91A872F.f04t02|Cabrita et al. (2007) Protein Science 16, 2360-2367]]))
  
 His-TEV plasmid - Birck His-TEV plasmid - Birck
Line 8: Line 16:
 pBS2440 optimized TEV plamsid - Seraphin pBS2440 optimized TEV plamsid - Seraphin
  
 +----
 +
 +==== Protocols ====
 +
 +[[purification:tev:W_protocol|Waugh protocol]]
 +
 +[[purification:tev:C_protocol|Cabrita protocol]]
  
 +[[purification:tev:B_protocol|Birck protocol]]
  
-[[purification:TEV:W_protocol|Waugh protocol]]+[[purification:tev:S_protocol|Seraphin protocol]]
  
-[[purification:TEV:B_protocol|Birck protocol]] 
  
-[[purification:TEV:S_protocol|Seraphin protocol]]+[[purification:tev:FAQ|TEV FAQ]]
purification/tev.1468422296.txt.gz · Last modified: (external edit)