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purification:tev

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purification:tev [2016/07/18 08:17] – bonnefonpurification:tev [2023/11/01 20:19] (current) – external edit 127.0.0.1
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 ===== In-house TEV production ===== ===== In-house TEV production =====
  
 +----
  
 ==== Clones ==== ==== Clones ====
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 [[https://www.addgene.org/8827/|pRK792 plasmid]] - His-TEV(S219V)-Arg - Waugh [[https://www.addgene.org/8827/|pRK792 plasmid]] - His-TEV(S219V)-Arg - Waugh
  
-**Sequence** +**Sequence**  
-<blockquote>G**HHHHHHH**GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMN**RRRRR**</blockquote>+<q>G**HHHHHHH**GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFM__V__KPEEPFQPVKEATQLMN**RRRRR**</q>
  
-His-TEV(L35V/S135G/S219V)-Arg - Cabrita+His-TEV(L35V/S135G/S219V)-Arg - Cabrita(([[http://onlinelibrary.wiley.com/doi/10.1110/ps.072822507/abstract;jsessionid=4269BED8C9677045F562FB78F91A872F.f04t02|Cabrita et al. (2007) Protein Science 16, 2360-2367]]))
  
 His-TEV plasmid - Birck His-TEV plasmid - Birck
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 pBS2440 optimized TEV plamsid - Seraphin pBS2440 optimized TEV plamsid - Seraphin
  
 +----
  
 ==== Protocols ==== ==== Protocols ====
purification/tev.1468829840.txt.gz · Last modified: (external edit)