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relion [2024/08/29 07:11] Luc Bonnefondrelion [2024/08/29 07:12] (current) Luc Bonnefond
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 <code>relion_estimate_gain --i Import/job001/movies.star --j 8 --max_frames 10000 --random true --o estimated_gain.mrc</code> <code>relion_estimate_gain --i Import/job001/movies.star --j 8 --max_frames 10000 --random true --o estimated_gain.mrc</code>
 Explanation of options: Explanation of options:
-  * <code>--j</code>: number of thread +  * <quote>--j</quote>: number of thread 
-  * <code>--max_frames</code>: target number of frames to average (rounded to movies) +  * <quote>--max_frames</quote>: target number of frames to average (rounded to movies) 
-  * <code>--random</code>: randomize the order of input movies before taking subset+  * <quote>--random</quote>: randomize the order of input movies before taking subset
  
 === Job Relion/Motion correction === === Job Relion/Motion correction ===
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 <code>relion_display --i Micrograph.mrc --scale 0.2 --lowpass 10 --angpix 0.862 --sigma_contrast 3</code> <code>relion_display --i Micrograph.mrc --scale 0.2 --lowpass 10 --angpix 0.862 --sigma_contrast 3</code>
 Explanation of options: Explanation of options:
-  * <code>--i</code>: Input; can be a single micrograph (.mrc) or a stack (.star), but not a movie (.eer, .tif) +  * <quote>--i</quote>: Input; can be a single micrograph (.mrc) or a stack (.star), but not a movie (.eer, .tif) 
-  * <code>--scale</code>: display size (0.2 = 20% of original) +  * <quote>--scale</quote>: display size (0.2 = 20% of original) 
-  * <code>--lowpass</code>: lowpass filtering (5 to 10 is a good range) +  * <quote>--lowpass</quote>: lowpass filtering (5 to 10 is a good range) 
-  * <code>--angpix</code>: angströms/pixel from the camera (0.862 for KriosI at x81000, 0.901 for KriosII and 0.729 for Glacios) +  * <quote>--angpix</quote>: angströms/pixel from the camera (0.862 for KriosI at x81000, 0.901 for KriosII and 0.729 for Glacios) 
-  * <code>--sigma_contrast</code>: contrast value to adjust (1 to 4, depending on the image, lowpass,...)+  * <quote>--sigma_contrast</quote>: contrast value to adjust (1 to 4, depending on the image, lowpass,...)
relion.txt · Last modified: by Luc Bonnefond