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titan_to_process [2024/06/19 10:57] – [3. Begin processing with relion] Luc Bonnefondtitan_to_process [2024/07/25 12:08] (current) – Luc Bonnefond
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 Estimate the gain with at least 200 movies (redo if needed with a larger number) Estimate the gain with at least 200 movies (redo if needed with a larger number)
-<code>relion_estimate_gain --i Import/job001/movies.star --j 8 --o estimated_gain.mrc</code>+<code>relion_estimate_gain --i Import/job001/movies.star --j 8 --max_frames 10000 --random true --o estimated_gain.mrc</code> 
 +Explanation of options: 
 +  * --j: number of thread 
 +  * --max_frames: target number of frames to average (rounded to movies) 
 +  * --random: randomize the order of input movies before taking subset
  
 === Job Relion/Motion correction === === Job Relion/Motion correction ===
    
 Specify the dose per frame, the number of patches 5x5m, provide the gain file, and set several MPI (number of tasks in parallel) and several (as a multiple of the number of frames in the movies) threads (number of cpu per task) Specify the dose per frame, the number of patches 5x5m, provide the gain file, and set several MPI (number of tasks in parallel) and several (as a multiple of the number of frames in the movies) threads (number of cpu per task)
titan_to_process.txt · Last modified: by Luc Bonnefond