====== Rules for cell culture room E2018 at CBI ======

Access to the room requires activation of your badge (it takes about a week) and a short introduction by Julien BATISSE.

==== How to book the medium for your production ? ====

The file “Prod Schedule for Media Order_2024” should be filled online (seafile) every time you make production : 
  * [[https://seafile.igbmc.fr/library/Lamour-Ruff_Common/|Prod Schedule for Media Order_2024]]
Please **fill the green lines** in this table. All the rest will be calculated automatically, allowing Julien to follow the remaining stocks.

{{:e2018_media_usage.png?600|}}

==== How to book the incubators ====

Book the incubators with the outlook calendar (ask Julien for the access)

==== What to order ? on which budget line ? ====

When something is missing , just go see Julien, he will tell you what to do.
  * routine staff (autoclave bags, paper…) => BSI com 
  * consumable staff (falcon, tips, stericup…) => teams 
  * **Good to know**: Julien has a « team » stock for « luna counting » slides and adhesive membranes (breathseal sealer). No need to order yourself, just go ask him.

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==== Reminder for media ====

=== Liquid GMEM ===

{{::gmem-bottle.jpg?100 |}}500 mL
  * + 30 mL BTP 30g/L (0,22µ filtred under PSM)
  * + 50 mL SVF
  * + 150 µL Gentamycin

=== GMEM powder ===

{{:gmem-vial.jpg?100|}} **vial for 1 L** or **bottle for 10 L** {{:gmem-bottle.jpg?100|}}

1 vial for 1 L culture: (or 1 bottle (for 10 L) weight up what you need)
  * Re-suspend in 900 mL sterilized mQ water (Denis)
  * + 60 mL BTP
  * + 30 mL Sodium Bicarbonate 90 g/L 
  * + 100 mL SVF
  * + 300 µL Gentamycin

{{:sodium_bicarbonate.jpg?100 |}}**Sodium Bicarbonate** : 
  * warm to 25°C for complete dissolution 
  * 0,22 µ filtered under PSM

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==== Cleaning ====

Please clean the PSM before and after your work with ummonium spray!

Don’t let your dirty bottles more than one day for decontamination (1 week-end, not more)

Cleaning sessions (each 3 weeks) has been  scheduled by Mylene
 
[[https://docs.google.com/spreadsheets/d/1f-fB5N8KR6xmudWqZ6yslXfAFT8ivqy3OuFtUXROMNs/edit?usp=sharing|schedule]]

{{:e2018-cleaning_schedule_202403.png|}}