===== Aimless =====

<code>
>setccp4
>ccp4i
</code>

Create a project in the /ccp4 subfolder

**Run Aimless**

  * If XDS was used before, choose the <q>Option to skip scaling & just merge</q> to prevent scaling a second time
  * If there are several datasets/scans for the same crystal scale and merge all sets in Aimless
  * If necessary choose <q>Customise symmetry determination>Choose a previous solution</q> to force the spacegroup
  * If necessary choose <q>Ensure unique data & add FreeR column...>Copy FreeR from another MTZ</q> to import the FreeR flags from a reference MTZ file
  * Specify the //Project//, //Crystal// and //Dataset// names
  * Change the output name to **drop_scaled1.mtz**
  * Make a second run in which you specify the high-resolution cut with <q>Exclude data resolution [...] greater than [ ] Angstroms</q> and name the output file **drop_res_scaled1.mtz**

**Run Sftools**

  * Use sftools to <q>delete columns</q> and remove the 13 unnecessary columns and name the output file **drop_res_scaled1_6col.mtz**
  * Use sftools to <q>delete reflections</q> and <q>Delete selected reflections:</q> where <q>Selection includes reflections with data missing in specificed column (F_drop)</q> and name the output file **drop_res_scaled1_6col_cleaned.mtz**