====== The Glacios microscope interfaces ======


===== The microscope console =====

**Location**: right in front of you on the desk

**Role**: it provides direct control of some of the most important microscope parts:
  * Magnification
  * Electron dose: spot size (C1 lens) and beam spread (C2 lens)
  * Focus (objective lens current)
  * Stage position (with the joystick and Z buttons)
  * Beam position (with the trackball)
  * and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs. 

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===== Gatan DigitalMicrograph User Interface (DM) =====

**Location**: On the left screen

**Role**: It controls the K2 direct electron detector.

  * The beginner user will not use it.
  * ++The expert user will use it to:|<div>
  * prepare Dark, linear and counting / Super Resolution gain references.
  * measure the dose.
  * monitor the K2 health status.
  * troubleshoot the system.
  * perform cryo-cycle of the detector
</div>++

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===== SerialEM User Interface (SEM) =====

**Location**: On the left screen

**Role**: It provides camera/microscope commands to allow the user to screen its grids and setup an automated data collection.

  * ++The beginner user will use it to:|<div>
  * record the Atlases.
  * select squares and prepare the square maps at eucentric height.
  * select targets (holes).
  * screen targets (holes).
</div>++
  * ++The expert user will use it to:|<div>
  * perform all the actions described above.
  * perform beam corrections (astigmatism, coma-free).
  * perform calibrations.
  * setup the dose rate, total dose and dose fractionning.
  * setup the time and imaging modes for the different beam modes.
  * center the different beam modes all together on the camera axis.
</div>++

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===== The TEM User Interface (TEM) =====

**Location**: On the middle screen

**Role**: It controls all the microscope systems.

  * ++The beginner user will use it to:|<div>
  * dock/undock the cassette from the NanoCab to the autoloader.
  * load/unload the grids in the column.
  * change and center the Condenser 2 and objective apertures.
  * control the autoloader turbo pump.
  * perform beam-shift adjustments.
  * manually set eucentric height.
</div>++
  * ++The expert user will use it to:|<div>
  * perform all the actions described above.
  * align the microscope.
  * troubleshoot the system.
  * manage the cold system.
  * perform cryo-cycle of the column/autoloader.
</div>++

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===== The FluCam User Interface (FC) =====

**Location**: On the right screen

**Role**: It allows the visualization of the electron beam and the sample image.

  * ++The beginner user will use it to:|<div>
  * center the beams.
  * center Condenser 2 and objective apertures.
  * look for and move to particular features on their grids.
  * manually set eucentric height.
</div>++
  * ++The expert user will use it to:|<div>
  * perform all the actions described above.
  * align the microscope.
</div>++