===== In-house TEV production =====

----

==== Clones ====

[[https://www.addgene.org/8827/|pRK792 plasmid]] - His-TEV(S219V)-Arg - Waugh

**Sequence** 
<q>G**HHHHHHH**GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFM__V__KPEEPFQPVKEATQLMN**RRRRR**</q>

His-TEV(L35V/S135G/S219V)-Arg - Cabrita(([[http://onlinelibrary.wiley.com/doi/10.1110/ps.072822507/abstract;jsessionid=4269BED8C9677045F562FB78F91A872F.f04t02|Cabrita et al. (2007) Protein Science 16, 2360-2367]]))

His-TEV plasmid - Birck

pBS2440 optimized TEV plamsid - Seraphin

----

==== Protocols ====

[[purification:tev:W_protocol|Waugh protocol]]

[[purification:tev:C_protocol|Cabrita protocol]]

[[purification:tev:B_protocol|Birck protocol]]

[[purification:tev:S_protocol|Seraphin protocol]]


[[purification:tev:FAQ|TEV FAQ]]