====== The TF20 microscope interfaces ====== 


===== The microscope console =====

**Location**: right in front of you on the desk

**Role**: It provides direct control of some of the most important microscope parts:
  * Magnification
  * Electron dose: spot size (C1 lens) and beam spread (C2 lens)
  * Focus (objective lens current)
  * Stage position (with the joystick and Z buttons)
  * Beam position (with the trackball)
  * and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs.

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===== Gatan DigitalMicrograph User Interface (DM) =====

**Location**: On the left screen

**Role**: It controls the US10001 Camera.

  * the user will use it to :
    * prepare Dark and gain references.
    * during screening, to acquire images in continuous mode.

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===== SerialEM User Interface (SEM) =====

**Location**: On the right screen

**Role**: It provides camera/microscope commands to allow the user to screen its grids and setup an automated data collection.

  * ++The beginner user will use it to:|<div>
  * record the Atlases.
  * select squares and prepare the square maps at eucentric height.
  * select targets (position).
  * screen targets at higher magnification (as single image or montage).
  * center the different beam modes all together on the camera axis.
</div>++
  * ++The expert user will use it to:|<div>
  * perform all the actions described above.
  * perform beam corrections (astigmatism, coma-free).
  * perform calibrations.  
</div>++

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===== The TEM User Interface (TUI) =====

**Location**: On left screen

**Role**: It controls all the microscope systems.


  * ++It is divided into 4 zones:|<div>

   *Zone1: TUI tabbed pages hosting different commands allowing to control the ME and to know its working state
   *Zone2: Window showing EM working state like HT value, stage position, magnification, etc …
   *Zone 3 : Additional TUI Tabbed page
   *Zone4 : Window defining functions of control panel button:
{{:capture2_tui_description.jpg?nolink&600|}}
</div>++
  * ++The beginner user will use it to:|<div>
  * load/unload the grids in the column.
  * change and center the Condenser 2 and objective apertures.
  * control the turbo pump.
  * perform beam-shift adjustments.
  * manually set eucentric height.
</div>++
  * ++The expert user will use it to:|<div>
  * perform all the actions described above.
  * align the microscope.
  * troubleshoot the system.
  * perform cryo-cycle of the column/autoloader.
</div>++


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===== The FluCam User Interface (FC) =====

**Location**: On the right screen

**Role:** It allows the visualization of the electron beam and the sample image.

  * ++The beginner user will use it to:|<div>
  * record the Atlases.
  * select squares and prepare the square maps at eucentric height.
  * select targets (position).
  * screen targets at higher magnification (as single image or montage).
  * center the different beam modes all together on the camera axis.
</div>++

  * ++The expert user will use it to:|<div>
  * record the Atlases.
</div>++