==== Make sure Microscope is in good shape (ppt associated) ==== *++ a.Make sure HT and FEG are on|
* On TEM User Interface (TUI) * setup tab > High Tension panel High tension button is highlighted yellow * setup tab > FEG Control panel Operate button is highlighted yellow {{ :capture_ht-operate-on.jpg?nolink |}}
++ *++ b.Make sure column valves are closed |
* On TEM User Interface (TUI) * setup tab > Vaccum panel Col. Valves closed button is highlighted yellow IMAGE
++ *++ c.Make sure that EM column vaccum is stable (IGP1 = 6)|
* On TEM User Interface (TUI) * setup tab > Vaccum panel Column value indicates 6 IMAGE
++ *++ d.Make sure stage is at neutral position |
* On TEM User Interface (TUI) * Stage2 tab > control panel press X,Y button to zero out the X, Y to center the stage IMAGE
++ - ++Setup the microscope for recording the Atlases|
* On TEM user interface (**TUI**).
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