Clipping Procedure

source : https://www.jonipsaro.com/post/krios/

• Fill a liquid nitrogen transfer dewars. Make sure it dry before beginning
• Assemble the necessary tools:
  • Flat-T autoloader forceps
  • C-clip holder wands (one per grid)
  • Rings and C-clips (one per grid)
  • Screwdriver
  • all part of the transfer station for grid clipping:
    • Table-top cryo dewar (and cover)
    • Aluminum & brass specimen transfer pedestal
    • circular platform aimed holding wands

• 1. Use forceps and firm pressure to insert a single C-clip into the wand.
  • Make sure that the C-clip is hugging the inside wall of the wand.
  • Use forceps to adjust if necessary.
• 2. To align the C-clip at the tip of the wand.
  • a. Hold the wand vertically by positionning it into the circular opening of the brass part of the transfer base.

    Inserer image

  • b. Press down on the wand trigger to engage and align the C-clip at the tip of the wand.
• 3. Inspect proper placement of the C-clip

  Arrange the transfer setup as pictured. It should include the specimen transfer pedestal, loaded C-clip wands, and autoloader forceps.
  Additionally, place clip rings (flat-side down) in each of the four mounting positions in the brass portion of the transfer pedestal.
  Inspect each clip ring to make sure that the clip side is face-up.
  Once clip rings are in place, rotate the brass disc such that all clips are covered. This will ensure they do not move while cooling the station.
  Place cryo-EM grid box(es) on the sample pedestal. Open them using the grid box fastener wand. Keep the grid box top attached to the fastner wand until needed.