The Glacios microscope interfaces

Location: right in front of you on the desk

Role: it provides direct control of some of the most important microscope parts:

• Magnification
• Electron dose: spot size (C1 lens) and beam spread (C2 lens)
• Focus (objective lens current)
• Stage position (with the joystick and Z buttons)
• Beam position (with the trackball)
• and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs.

Location: On the left screen

Role: It controls the K2 direct electron detector.

• The beginner user will not use it.
• The expert user will use it to:

  • prepare Dark, linear and counting / Super Resolution gain references.
  • measure the dose.
  • monitor the K2 health status.
  • troubleshoot the system.
  • perform cryo-cycle of the detector

Location: On the left screen

Role: It provides camera/microscope commands to allow the user to screen its grids and setup an automated data collection.

• The beginner user will use it to:

  • record the Atlases.
  • select squares and prepare the square maps at eucentric height.
  • select targets (holes).
  • screen targets (holes).

• The expert user will use it to:

  • perform all the actions described above.
  • perform beam corrections (astigmatism, coma-free).
  • perform calibrations.
  • setup the dose rate, total dose and dose fractionning.
  • setup the time and imaging modes for the different beam modes.
  • center the different beam modes all together on the camera axis.

Location: On the middle screen

Role: It controls all the microscope systems.

• The beginner user will use it to:

  • dock/undock the cassette from the NanoCab to the autoloader.
  • load/unload the grids in the column.
  • change and center the Condenser 2 and objective apertures.
  • control the autoloader turbo pump.
  • perform beam-shift adjustments.
  • manually set eucentric height.

• The expert user will use it to:

  • perform all the actions described above.
  • align the microscope.
  • troubleshoot the system.
  • manage the cold system.
  • perform cryo-cycle of the column/autoloader.

Location: On the right screen

Role: It allows the visualization of the electron beam and the sample image.

• The beginner user will use it to:

  • center the beams.
  • center Condenser 2 and objective apertures.
  • look for and move to particular features on their grids.
  • manually set eucentric height.

• The expert user will use it to:

  • perform all the actions described above.
  • align the microscope.