The TF20 microscope interfaces

Location: right in front of you on the desk

Role: It provides direct control of some of the most important microscope parts:

• Magnification
• Electron dose: spot size (C1 lens) and beam spread (C2 lens)
• Focus (objective lens current)
• Stage position (with the joystick and Z buttons)
• Beam position (with the trackball)
• and many other actions through the programmable switches (L/R1-2-3) and Multifunction X/Y knobs.

Location: On the left screen

Role: It controls the US10001 Camera.

• the user will use it to :
  • prepare Dark and gain references.
  • during screening, to acquire images in continuous mode.

Location: On the right screen

Role: It provides camera/microscope commands to allow the user to screen its grids and setup an automated data collection.

• The beginner user will use it to:

  • record the Atlases.
  • select squares and prepare the square maps at eucentric height.
  • select targets (position).
  • screen targets at higher magnification (as single image or montage).
  • center the different beam modes all together on the camera axis.

• The expert user will use it to:

  • perform all the actions described above.
  • perform beam corrections (astigmatism, coma-free).
  • perform calibrations.

Location: On left screen

Role: It controls all the microscope systems.

• It is divided into 4 zones:

  • Zone1: TUI tabbed pages hosting different commands allowing to control the ME and to know its working state
  • Zone2: Window showing EM working state like HT value, stage position, magnification, etc …
  • Zone 3 : Additional TUI Tabbed page
  • Zone4 : Window defining functions of control panel button:

  

• The beginner user will use it to:

  • load/unload the grids in the column.
  • change and center the Condenser 2 and objective apertures.
  • control the turbo pump.
  • perform beam-shift adjustments.
  • manually set eucentric height.

• The expert user will use it to:

  • perform all the actions described above.
  • align the microscope.
  • troubleshoot the system.
  • perform cryo-cycle of the column/autoloader.

Location: On the right screen

Role: It allows the visualization of the electron beam and the sample image.

• The beginner user will use it to:

  • record the Atlases.
  • select squares and prepare the square maps at eucentric height.
  • select targets (position).
  • screen targets at higher magnification (as single image or montage).
  • center the different beam modes all together on the camera axis.

• The expert user will use it to:

  • record the Atlases.