• a.Make sure HT and FEG are on

  • On TEM User Interface (TUI)
  • setup tab > High Tension panel High tension button is highlighted yellow
  • setup tab > FEG Control panel Operate button is highlighted yellow

  

• b.Make sure column valves are closed

  • On TEM User Interface (TUI)
  • setup tab > Vaccum panel Col. Valves closed button is highlighted yellow

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• c.Make sure that EM column vaccum is stable (IGP1 = 6)

  • On TEM User Interface (TUI)
  • setup tab > Vaccum panel Column value indicates 6

  IMAGE

• d.Make sure stage is at neutral position

  • On TEM User Interface (TUI)
  • Stage2 tab > control panel press X,Y button to zero out the X, Y to center the stage

  IMAGE

- Setup the microscope for recording the Atlases