glacios_alignment
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Table of Contents
Basic Microscope Alignment
The microscope should be roughly aligned when you arrive.
There is no need to do a complete alignment every session, if the microscope behaves strangely, ask for help!
You should perform only two or three steps:
- Camera gain reference
- Direct alignment
- Objective Astigmatism / Coma-free alignment
Gain reference of the K2 camera
This is done in Digital Micrograph on the K2 server.
Direct alignment
This is done in microscope interface.
- Insert calibration specimen (position 1 in the autoloader)
- Find an unbroken square
- Bring sample to eucentric high
- Press Eucentric Focus on the right control panel
Open the Direct alignment tab
DO NOT TOUCH GUN TILT/SHIFT
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