• Introduce the Cross Grating Grid in the column.
• Go to x34 Imaging status.
• Select 2 positions: a carbon square and an empty square.
• Introduce the Condenser 2 of 30 um
• Go to x280 Imaging status.
• In DigitalMicrograph click View and place the mouse arrow on a feature.
• Use Alpha Toogle 20° in Stage² window (TEM) to rock the grid.
• Correct with Z axis knobs on the console.
• Repeat a second time.
• Go to x45k Imaging status
• In Low Dose Control window (SEM) click on Go to: Record.
• Push the Eucentric Focus knob on the console.
• Inset the screen (FC)
• Push the Wobbler knob on the console
• Adjust the Z height on the screen with the Z axis knobs on the console.

Microprobe

• Go to microprobe (TEM)
• Push the Eucentric Focus knob on the console.
• In the Direct Alignments window:
• Click on Beam tilt pp X.
• Adjust with Multifunction X/Y knobs.
• Click Done at the bottom of the window.
• Click on Beam tilt pp Y.
• Adjust with Multifunction X/Y knobs.
• Click Done at the bottom of the window.
• Click on Beam shift.
• Adjust with Multifunction X/Y knobs.
• Click Done at the bottom of the window.
• Click on Rotation center.
• Adjust with Multifunction X/Y knobs.
• Click Done at the bottom of the window.