• Introduce the Cross Grating Grid in the column.
• Go to x34 Imaging status.
• Select 2 positions: a carbon square and an empty square.
• Introduce the Condenser 2 of 30 um
• Go to x280 Imaging status.
• In DigitalMicrograph click View and place the mouse arrow on a feature.
• Use Alpha Toogle 20° in Stage² window (TEM) to rock the grid.
• Correct with Z axis knobs on the console.
• Repeat a second time.
• Go to x45k Imaging status
• In Low Dose Control window (SEM) click on Go to: Record.
• Push the Eucentric Focus knob on the console.
• Inset the screen (FC).
• Push the Wobbler knob on the console.
• Adjust the Z height on the screen with the Z axis knobs on the console.

Microprobe

Focus the beam and center it (with the trackball).

• Go to microprobe (TEM)
• Push the Eucentric Focus knob on the console.
• In the Direct Alignments window:

1. Adjust the Beam tilt pp X

   • Click on Beam tilt pp X
   • Adjust with Multifunction X/Y knobs
   • Click Done at the bottom of the window.

2. Adjust the Beam tilt pp Y

   • Click on Beam tilt pp Y.
   • Adjust with Multifunction X/Y knobs.
   • Click Done at the bottom of the window.

3. Adjust the Beam shift

   • Click on Beam shift.
   • Adjust with Multifunction X/Y knobs.
   • Click Done at the bottom of the window.

4. Adjust the Rotation center

   • Click on Rotation center.
   • Adjust with Multifunction X/Y knobs.
   • Click Done at the bottom of the window.

Nanoprobe

Focus the beam and center it (with the trackball).

• Go to nanoprobe (TEM)
• Push the Eucentric Focus knob on the console.
• Check the Condenser 2 centering and adjust if necessary.
• In the Direct Alignments window:

1. Adjust the Beam tilt pp X

   • Click on Beam tilt pp X
   • Adjust with Multifunction X/Y knobs
   • Click Done at the bottom of the window

2. Adjust the Beam tilt pp Y

   • Click on Beam tilt pp Y
   • Adjust with Multifunction X/Y knobs.
   • Click Done at the bottom of the window

3. Adjust the Beam shift

   • Click on Beam shift
   • Adjust with Multifunction X/Y knobs
   • Click Done at the bottom of the window

4. Adjust the Rotation center

   • Click on Rotation center
   • Adjust with Multifunction X/Y knobs
   • Click Done at the bottom of the window

After all calibrations save the settings