https://bsi.inscog.eu/ 2026-06-01T21:59:09+00:00 https://bsi.inscog.eu/ https://bsi.inscog.eu/lib/exe/fetch.php?media=wiki:dokuwiki.svg text/html 2023-11-01T20:19:12+00:00 Anonymous (anonymous@undisclosed.example.com) https://bsi.inscog.eu/doku.php?id=crystallization:cryoprotectants&rev=1698869952&do=diff Cryoprotectants List of cryprotectants used successfully in flash–cooling macromolecular crystals Type Concentration (%) Glycerol 13–30(v/v) Ethylene glycol 11–30(v/v) Polyethylene glycol 400 25–35(v/v) Xylitol 22(w/v) (2R,3R)–(–)–butane–2,3–diol text/html 2023-11-01T20:19:10+00:00 Anonymous (anonymous@undisclosed.example.com) https://bsi.inscog.eu/doku.php?id=crystallization:insitu_proteolysis&rev=1698869950&do=diff In situ proteolysis [1] Dong et al. (2007) In situ proteolysis for protein crystallization and structure determination. Nature Methods 4:1019. [2] Wernimont et al. (2009) In Situ Proteolysis to Generate Crystals for Structure Determination: An Update. PLoS ONE 4:e5094 text/html 2023-11-01T20:19:12+00:00 Anonymous (anonymous@undisclosed.example.com) https://bsi.inscog.eu/doku.php?id=crystallization:lysine_methylation&rev=1698869952&do=diff Methylation Protocol Walter TS, Meier C, Assenberg R, Au K, Ren J, Verma A, Nettleship JE, Owens RJ, Stuart DI, Grimes J Lysine methylation as routine rescue strategy for crystallization Structure WAYS & MEANS 2006 Nov;14(11):1617-22. Chemicals: *