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glacios_screening [2023/01/20 17:09] – [1. Load the Grids] crucifixglacios_screening [2023/11/01 20:18] (current) – external edit 127.0.0.1
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-==== 1.Make sure Microscope is in good shape (ppt associated)==== 
-  - a.Make sure HT and FEG are on 
- * Under « setup » tab,  « high tension » and « operate » are highlighted yellow 
-b. Make sure that column valves are closed and turbo pump is on 
-i. Under « setup » tab,  « col. Valves closed » and « Turbo on » are highlighted yellow 
-c. Make sure that EM column vaccum is stable (IGP1 -6) 
-d. Make sure that Stage neutral 
-i. Under « Search » tab 
-1. click « X,Y » button  to zero out the X, Y to center the stage 
-2. If necessary Click « AB » button  to zero out   coordinates of the stage 
-e. Make sure cold trap already with LN2, refill every 3-4 hours 
  
-2. Load grid in holder 
-a. Place holder in stand with spring holder up 
-b. Lift (open) spring holder using metal pin 
-c. Center specimen grid in holder socket 
-d. d. Close spring holder using metal pin 
  
  
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   - ++Load platform or user settings|<div>   - ++Load platform or user settings|<div>
-  * Menu: //Settings > Open > SerialEM_Settings_SPA//+  * Menu: //Settings > Open > Platform_Settings_SPA//
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glacios_screening.1674234593.txt.gz · Last modified: (external edit)